Effect of IRS4 Levels on PI 3-Kinase Signalling (original) (raw)
Figure 6
Deletion of PH domain S4 targets IRS4 to the nucleus.
U2OS-Flpin™ cells expressing full length IRS4-FLAG and the indicated fragments containing residues from 200–end-IRS4-FLAG or 336–end-IRS4-FLAG proteins were analysed for their subcellular localization, under serum starved (SS) or addition of IGF1(50 ng/ml, 20 min). Cells were stained for FLAG antibody and with DAPI as described in Materials and Methods.