MLL Becomes Functional through Intra-Molecular Interaction Not by Proteolytic Processing (original) (raw)
Figure 2
Intra-molecular interaction is required for expansion of hematopoietic progenitors.
A. Cellularity of de mutant FLs. Error bars represent the standard deviations of cell numbers of FLs. For each genotype, at least 3 livers were analyzed. B. Population analysis of de mutant FLs by flow cytometry. The fetal LKS compartment was defined as the Lin−cKit+Sca-1+Mac1+ population. C. Frequency of LKS populations in de mutant FLs. Error bars represent the standard deviations of more than two littermate embryos for each genotype. D. Population analysis of HSCs from MPPs in de mutant FLs by flow cytometry. Lin−Mac1+ cells were subdivided by Sca-1 and CD48. E. Frequencies of HSCs (top) and MPPs (bottom) in de mutant FLs. Error bars represent the standard deviations of more than four embryos for each genotype. Frequency was expressed as the percentage of each fraction within the whole population. F. Ability of de mutant FL cells to reconstitute the hematopoietic system. 5×105 cells of de homozygous FLs and 5×104 cells of the wild type/heterozygous controls were transplanted into lethally irradiated syngenic mice. Previously published data for dC homozygous FLs [8] obtained in the same experimental setting were included for comparison. N: the number of FLs analyzed.