MLL Becomes Functional through Intra-Molecular Interaction Not by Proteolytic Processing (original) (raw)
Figure 6
MLL processing is not required for proliferation of oncogene-transformed myeloid progenitors.
A. Experimental scheme of myeloid progenitor serial replating assay. Hoxa9 was transduced in Figure 6B and MLL-AF9 in Figure 6C and 6D. The time points at which colony forming units and Hoxa9 expression were measured are shown. B. Clonogenic potentials of _Hoxa9_-transformed myeloid progenitors of wild type or uc homozygous mutant origin. CFUs per 104 plated cells were enumerated at third and fourth round. Error bars represent standard deviations of three independent samples. C. Clonogenic potentials of _MLL-AF9_-transformed myeloid progenitors of wild type or uc homozygous mutant origin. CFUs per 104 plated cells were enumerated at third and fourth round. Error bars represent standard deviations of three independent samples. D. Hoxa9 expression in the first round colonies of _MLL-AF9_-transformed cells. Relative expression levels (normalized to Gapdh) and expressed relative to those of _MLL-AF9_-transformed wild type progenitors arbitrarily set as 100%. Error bars represent standard deviations of triplicate PCRs.