Commensal Akkermansia muciniphila Exacerbates Gut Inflammation in Salmonella Typhimurium-Infected Gnotobiotic Mice (original) (raw)
Figure 6
SIHUMI mice colonized with both A. muciniphila and S. Typhimurium display an increased cecal macrophage infiltration.
(A) Formalin fixed paraffin embedded cecum tissue was thin sectioned at 2 µm. Macrophages were stained by targeting the F4/80 receptor expressed on mouse macrophages using immunohistochemistry with specific antibodies. Brown color indicates positively stained macrophages. Magnification 400-fold. Bar = 100 µm. (B) Positively stained macrophages were enumerated along a stretch of 50 µm of lamina muscularis for both lamina propria and sub-mucosa (see materials and methods). SIHUMI mice colonized with both A. muciniphila and S. Typhimurium had the highest macrophage infiltration scores compared to the other groups (see Figure. 1). Data are expressed as median with range. n = 5 mice per group. *P<0.05, **P<0.01, ***P<0.001. Amuc: A. muciniphila; S. Tm: S. Typhimurium.