The Small Molecule Wnt Signaling Modulator ICG-001 Improves Contractile Function in Chronically Infarcted Rat Myocardium (original) (raw)
Figure 4
Effects of IQ1 on mouse embryonic hearts.
(A) Mouse embryos were exposed to IQ1 from E12.5 to E13.5 in utero. Edematous skin is observed in IQ1 treated embryos (arrow). (B) Embryos were exposed to IQ1 from E12.5 to E16.5. IQ1 reduced the size of the embryonic hearts, size of ventricular spaces and number of trabeculae in the ventricles in transverse H-E sections (top). Whole mount PECAM-1 immunostaining shows the coronary vascular network from a dorsal view of the hearts (arrows, bottom). IQ1 decreased the number of blood vessels. LV; left ventricle, RV; right ventricle. (C) Mouse embryos were exposed to IQ1 from E12.5 to E14.5 in utero. β-galactosidase activity (blue) shows _Wt1_-derived cells in transverse heart sections. Nuclei were counterstained with Nuclear Fast Red. (D) Wt1-derived cells were quantified by blue pixels. IQ1 treatment decreased _Wt1_-derived cells in mouse embryonic hearts significantly. *P = 0.0197. Data are presented as mean ± s.e.m.