The Small Molecule Wnt Signaling Modulator ICG-001 Improves Contractile Function in Chronically Infarcted Rat Myocardium (original) (raw)
Figure 6
Histology and gene expression analysis of ICG-001 treated rats from the myocardial infarction model.
(A) Photomicrographs of transverse sections of the hearts at 10 days post-surgery. ICG-001 did not histologically change the hearts at the non-infarcted areas and the border zone by H-E staining (top and middle panel) and Gomori’s trichrome staining (bottom panel). Red; muscle, blue green; collagen, dark purple; hematoxylin/nuclear stain. Contribution of epicardium to cardiac regeneration. (B) Continuous administration of BrdU labeled replicating cells in the adult rat hearts at 7 days post-coronary occlusion. Brown; BrdU positive staining, dark purple; hematoxylin/nuclear stain. BrdU positive nuclei were found in cardiomyocytes near the epicardium (top and middle panel). BrdU positive nuclei occupy the center of the cross-sectioned cardiomyocytes (arrows). BrdU positive nuclei were also found in granulation tissue at the border zone but not in cardiomyocytes (bottom panel). (C) qPCR analysis of hearts at 7 and 10 days post-coronary occlusion in adult rats. ICG-001 showed a trend toward increased expression of Gdf15, Kit, Sdf1 and Fgf2 at both day7 and day10, but statistical significance was not reached (_P_>0.05). Data are presented as mean ± s.e.m.