Gfi1 and Gfi1b Repress Rag Transcription in Plasmacytoid Dendritic Cells In Vitro (original) (raw)
Figure 4
Deleting Gfi1 and Gfi1b does not result in a change in lymphoid- or pDC-specific genes in pDCs.
(A) RT-PCR of lymphoid-specific gene expression in ex vivo differentiated B220+ CD11c+ pDCs from Gfi1 f/f ; Gfi1b f/f ; ERCre mice untreated (WT) and treated (KO) with tamoxifen. RNA isolated from primary B (B) or T (T) cells were used as controls. (B) Quantitative RT-PCR of pDC-specific genes in ex vivo differentiated B220+ CD11c+ pDCs from Gfi1 f/f ; Gfi1b f/f ; ERCre mice untreated (WT) and treated (KO) with tamoxifen. Expression values in arbitrary units are normalized to Hprt transcript abundance.