STAT1 Regulates Human Glutaminase 1 Promoter Activity through Multiple Binding Sites in HIV-1 Infected Macrophages (original) (raw)
Figure 4
Regulation of rat GSL1 promoter activity by STAT1 binding sites.
(A) Two STAT1 putative binding sites for rat Gls1 promoter and the derived mutants for these sites were listed based on their distance (base pairs) upstream from the transcription start site. (B) Schematic representation of the various promoter mutant luciferase constructs and their activities in dual-luciferase assay. Wild type or the mutants of rat Gls1 promoter construct-transfected cells were treated with or without 100 U/ml IFN-α for 24 hours. Luciferase promoter activity assays were performed as described in Figure 1. The data are representative of three independent experiments and are the means of triplicate samples. *, p < 0.05 when compared with the wild type.