Melanotic Pathology and Vertical Transmission of the Gut Commensal Elizabethkingia meningoseptica in the Major Malaria Vector Anopheles gambiae (original) (raw)
Figure 2
Induction of lesions by injection of melanotic tissues in Anopheles species.
(A) Melanotic fat body tissues induce lethality in injected mosquitoes. Adult females were injected with abdomen-derived fat body tissues of melanotic lesion-bearing or of control adult females. Melanotic fat body extracts (FBE) were injected using two dilutions (1XFBE crude extract or 10-fold diluted extract 0.1XFBE). Survival rate was monitored daily, and represented as bars with standard error, as a percentage for each group. Three biologically independent injection assays were performed with 15-20 mosquitoes per condition. Points indicated by *** are very highly significantly different (P<0.05) between FBE control and 1XFBE or 0.1XFBE values.(B) Light micrographs of fat body tissues derived from FBE-injected female mosquitoes. Melanotic lesions in An. gambiae (upper panel; scale bar 0.05mm), and An. stephensi (lower panel; scale bar 0.5mm) mosquito species, resembling melanotic aggregates in the fat body of the affected ‘donor’ mosquito (see Figure 1).