Ashwagandha (Withania somnifera) Reverses β-Amyloid1-42 Induced Toxicity in Human Neuronal Cells: Implications in HIV-Associated Neurocognitive Disorders (HAND) (original) (raw)
Figure 7
Confocal Images of DIL stained SK-N-MC cells showing the effect of HIV-1Ba-L (clade B), Ashwagandha and Ashwagandha plus HIV-1Ba-L (clade B).
1. A. Control, B. HIV-1Ba-L (clade B) treated, C. Ashwagandha treated and D. Ashwagandha plus HIV-1Ba-L (clade B) treated. 2. Quantitative analysis showing the decreased spine density in HIV-1Ba-L (clade B) treated SK-N-MC cell line and its reversal by Ashwagandha (ASH). SK-N-MC cells were grown onto the glass coverslips, DIL stained and observed under confocal microscope. Randomly selected pictures in each group of the cells were captured in confocal microscope. Image J software was used to analyze the spine density, spine area, spine length and number of spines.