Single Nucleotide Polymorphisms of Human STING Can Affect Innate Immune Response to Cyclic Dinucleotides (original) (raw)
Figure 5
Western blot analysis of p-IRF3 level in response to cyclic dinucleotides.
293T cells transiently transfected to express STING or the SNPs as described in the Methods were harvested and the lysates were probed with antibody to detect p-IRF3 and STING. The loading control (LC) was a non-specific band that cross-reacted with the p-IRF3 antibody. The ratio of the signal present in the p-IRF3 divided by the signal in the LC for the WT STING was normalized to 100%.