Single Nucleotide Polymorphisms of Human STING Can Affect Innate Immune Response to Cyclic Dinucleotides (original) (raw)
Figure 6
Mutational analysis of substitution in HAQ variant on c-di-GMP or 3’3’cGAMP-dependent IFN-β signaling.
(A) Effects of substitutions on c-di-GMP-mediated IFN-β signaling. About 2 ng of each plasmid was transfected into 293T cells for 24h and then the cells were stimulated with 20 μg/ml of c-di-GMP. The ratio of IFN-β signaling stimulated with c-di-GMP via unstimulated was plotted as relative IFN-β fold induction. (B) Effects of substitutions on 3’3’cGAMP-mediated IFN-β signaling. (C) Effects of c-di-GMP concentration on the stimulation of IFN-β activity by hSTING and mutants. The cells were transfected with 2 ng of WT hSTING or mutant plasmid together with IFN-β reporter and further stimulated with different concentration of c-di-GMP at 24h posttransfection. (D) Effects of 3’3’cGAMP concentration on the stimulation of IFN-β signaling.