MicroRNA-26a Inhibits Angiogenesis by Down-Regulating VEGFA through the PIK3C2α/Akt/HIF-1α Pathway in Hepatocellular Carcinoma (original) (raw)
Figure 2
The anti-angiogenic effect of miR-26a is mainly though regulation of the PI3K/Akt/HIF/VEGFA pathway in HepG2 cells.
HIF-1α inhibitor, YC-1, PI3K inhibitor, and LY294002 were used to treat HCC cells. (A) The expression of VEGFA, Akt, p-Akt, and HIF-1α in all HCC cells. (B) After YC-1 or LY294002 was used to treat HepG2 cells with or without transfection of miR-26a, the expression of VEGFA was assessed by Western blot and ELISA. (C, D) After YC-1 or LY294002 was used to treat HepG2 cells, CM was collected and the effect of CM on HUVEC tube formation and migration was assessed. The tube length and the number of migrating cells were evaluated by counting 10 random fields at ×100 magnification. * compare to HepG2-wt, P < 0.05; # compare to HepG2-control, P < 0.05; ^ compare to HepG2-miR-26a, P < 0.05.