Methylglyoxal Evokes Pain by Stimulating TRPA1 (original) (raw)
Figure 7
TRPA1 does not influence insulin release.
(A) MG (0.5 mM) evokes indistinguishable [Ca2+]i-responses in β-cells from Trpa1+/+ (black) and Trpa1−/− (red) mice (n = 15-28). (B) Illustration of the time course of MG and tolbutamide (200 µM) evoked [Ca2+]i-responses in β-cells. (C, D) Effect of the TRPA1 antagonist HC030031 (HC, 100 mg/kg) or vehicle (Veh) administered 30 min before glucose or vehicle in the glucose tolerance test (2 g/kg, i.p.) performed in Trpa1+/+ (C) and Trpa1−/− mice (D). Data points are mean ± s.e.m of n = 6 mice and were analyzed by ANOVA followed by Tukey’s HSD test (*P<0.05, **P<0.01, ***P<0.001 compared to the group treated with vehicle and glucose, †P<0.05 compared to the group treated with vehicle and vehicle).