Alternative Splicing of the RAGE Cytoplasmic Domain Regulates Cell Signaling and Function (original) (raw)
Figure 3
RAGEΔICD affects RAGE-ligand signaling.
A. C6 glioma cells expressing RAGE or RAGEΔICD were stimulated with 5 ug/ml S100B for 5 minutes. Cell lysate was subjected to Western blot for antibodies against phospho-ERK1/2, p38 and SAPK/JNK. GAPDH was detected in total cell lysate by Western blot using monoclonal anti-GAPDH for normalization. B–D. Quantification of triplicate repeats normalized to GAPDH was performed for pERK (B), p38 (C), and pJNK, where *, p<0.05 and **, p = 0.005.