Alternative Splicing of the RAGE Cytoplasmic Domain Regulates Cell Signaling and Function (original) (raw)

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Figure 3

RAGEΔICD affects RAGE-ligand signaling.

A. C6 glioma cells expressing RAGE or RAGEΔICD were stimulated with 5 ug/ml S100B for 5 minutes. Cell lysate was subjected to Western blot for antibodies against phospho-ERK1/2, p38 and SAPK/JNK. GAPDH was detected in total cell lysate by Western blot using monoclonal anti-GAPDH for normalization. B–D. Quantification of triplicate repeats normalized to GAPDH was performed for pERK (B), p38 (C), and pJNK, where *, p<0.05 and **, p = 0.005.

Figure 3

doi: https://doi.org/10.1371/journal.pone.0078267.g003