Notch-1 Signaling Regulates Microglia Activation via NF-κB Pathway after Hypoxic Exposure In Vivo and In Vitro (original) (raw)

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Figure 2

Notch signaling was activated in primary cultured microglia exposed to hypoxia.

(A) Immunofluorescence images showing NICD expression in primary microglia labeled with lectin (a, e; green). The expression is intensely augmented both in the cytoplasm and nucleus after hypoxic treatment for 12 h (f, g) compared with the control (b, c). (B) Reverse transcription (RT)-PCR analysis of RBP-Jκ and Hes-1 mRNA expression in primary microglia exposed to hypoxia for 2, 4, 6, 12 and 24 h and control (c). Note the significant increase in RBP-Jκ and Hes-1 mRNA expression after hypoxia. The values represent the mean ±SD in triplicate. Significant differences between control and hypoxic BV-2 cells are expressed as *p<0.05 and ** p<0.01. Scale bars = 50 µm (A).

Figure 2

doi: https://doi.org/10.1371/journal.pone.0078439.g002