Tension Monitoring during Epithelial-to-Mesenchymal Transition Links the Switch of Phenotype to Expression of Moesin and Cadherins in NMuMG Cells (original) (raw)
Figure 2
Scheme showing (A) AFM indentation and (B) tether pulling experiments before and after the epithelial-to-mesenchymal transition (EMT).
The dashed arrows indicate the direction of force exerted to the membrane cortex upon either indentation or pulling. (A) Force indentation curves are analyzed using a tension model model [28], [30], [43]. Exemplarily chosen force-distance curve (red) and the corresponding fit by applying the extended indentation model (blue). Whereas T0 dominates the elastic response upon low indentation depths, the influence of KA increases at higher indentations a lateral stretching of the cellular shell occurs. A typical AFM force-distance curve of an untreated NMuMG cell including cantilever approach is shown (red). (B) During cantilever retraction (blue) the characteristic formation of a tether at constant force is observable at about 1.5 µm away from the surfaces.