Automated Cell Tracking and Analysis in Phase-Contrast Videos (iTrack4U): Development of Java Software Based on Combined Mean-Shift Processes (original) (raw)
Figure 5
Representativeness of the tracked cells.
Cells were imaged every four minutes for 12 hours and experiments were repeated three times. All cells that were lost during the automatic tracking were re-analyzed by the manual method. We compared the following variables for the population of cells followed by the software (41 cells) and the population of cells lost by the software (26 cells), both populations being analyzed manually here.
A. Total distance of migration by WM852 human melanoma cells. The two cell populations did not show any statistically significant difference (standard unpaired t-test, p = 0.5756).
B and C. Average distance of migration by WM852 human melanoma cells. The two cell populations did not show any statistically significant difference (standard unpaired t-test, p = 0.5757 and p = 0.5698 for the average distance and standard deviation, respectively).
D. Maximum distance of WM852 human melanoma cells. The two cell populations did not show any statistically significant difference (standard unpaired t-test, p = 0.4433).
E. Persistence of migration by WM852 human melanoma cells. The two cell populations did not show any statistically significant difference (standard unpaired t-test, p = 0.5632).
F. Pause of migration by WM852 human melanoma cells. The two cell populations did not show any statistically significant difference (standard unpaired t-test, p = 0.6459).
G and H. Average migration speed of WM852 human melanoma cells. The two cell populations did not show any statistically significant difference (standard unpaired t-test, p = 0.5953 and p = 0.5057 for the average speed and standard deviation, respectively).
I. Average acceleration of migration by WM852 human melanoma cells. The two cell populations did not show any statistically significant difference (standard unpaired t-test, p = 0.6894).