The Gene Expression Profile of Peripheral Blood Mononuclear Cells from EV71-Infected Rhesus Infants and the Significance in Viral Pathogenesis (original) (raw)
Figure 5
Up-regulation of the IL-17 gene in lung tissues from EV71-infected rhesus monkey infants.
(a) The fold-change of IL-17 gene expression in the PBMCs from EV71-infected neonatal rhesus monkeys according to microarray analysis. The color scale indicates the levels of gene expression from low (green, blue and purple) to high (red, pink and yellow). The values are shown on a log2 scale. (b) The levels of IL-17 gene expression in the lungs of the EV71-infected neonatal rhesus monkeys were measured by real-time qPCR. The y-axis indicates the relative quantity of the IL-17 mRNA in the samples compared with the control (Y = 1), and normalized to the endogenous GAPDH expression level. The samples were collected at days 4, 7 and 10 p.i.. Individual detection was performed in triplicate. Error bars are presented as the mean±SD. (c) The expression of IL-17 in lung tissues was measured by an immunohistochemical assay. The samples were tested with an anti-IL17A antibody and HRP-conjugated anti-mouse IgG antibody. Images are shown at 400× magnification.