Ubiquitin B in Cervical Cancer: Critical for the Maintenance of Cancer Stem-Like Cell Characters (original) (raw)
Figure 3
HeLa/TSA cells were enriched of cancer stem-like cells.
A, HeLa/TSA mammosphere cells were analyzed for the Sox2, Oct4 and Nanog mRNA levels. The columns represent the average of three separate experiments; error bars, SD. B, The quantitative analysis of Sox2, Oct4, Nanog, Mdr-1 and UbB protein levels by western blotting. C, HeLa/TSA mammosphere cells were analyzed by immunofluorescence for Sox2, Oct4 and Nanog. The representative photographs were taken using a fluorescence microscope (original magnification, ×200). The nuclei were presented as detected using DAPI (4′, 6-Diamidino-2-phenylindole) staining. D, The analysis of the side-population cell fraction in HeLa and HeLa/TSA cell cultures. Left panel: HeLa; right panel: HeLa/TSA. E, The analysis of CD44 and CD24 expression of HeLa and HeLa/TSA cells. Left panel: HeLa; right panel: HeLa/TSA.