Pleiotropic Effects of Blastocystis spp. Subtypes 4 and 7 on Ligand-Specific Toll-Like Receptor Signaling and NF-κB Activation in a Human Monocyte Cell Line (original) (raw)
Figure 3
Blastocystis effects on zymosan (ZG) stimulation of THP1-Blue monocytes.
Detection of SEAP activity from cell culture supernatants of THP1-Blue monocytes incubated with various conditions. A, B. Both Blastocystis ST7-B and ST4-WR1 significantly inhibits ZG-induced activation of THP1-Blue monocytes, as observed in the decrease in SEAP activity. Parasite lysate was added to THP1-Blue cells in terms of amounts of lysate prepared from 1 million (mil), 5 million, or 10 million parasites per ml. Inhibition observed to be in negative correlation with parasite lysate concentration, with significance observed from 5×106 ST7-B/ml onward. **, p<0.01, when analyzed against 5 µg/ml ZG positive control.