Partial Methylation at Am100 in 18S rRNA of Baker's Yeast Reveals Ribosome Heterogeneity on the Level of Eukaryotic rRNA Modification (original) (raw)
Figure 1
Identification of 18S rRNA molecules in wild type (WT) and Δ_snr51_ that lack the 2′-_O_-ribose methyl group at nucleotide A100.
A) Detection of 18S rRNA molecules lacking the Am100 modification by DNAzyme cleavage. Total RNA from wild type and Δ_snr51_ cells grown in YEPD medium to exponential phase was incubated with or without the DNAzyme 10-23-snR51-A100 and afterwards analyzed by gel analysis. The RNA band marked with an asterisk represents the large 18S rRNA fragment after cleavage at nucleotide A100. The 100 nucleotide fragment ran out of the gel. B) Schematic illustration of DNAzyme 10–23-snR51-A100 binding to its target site in 18S rRNA. The cleavage site 3′ to A100 is marked with an arrow. The resulting fragments have lengths of 100 nt and 1700 nt.