The Synthetic α-Bromo-2′,3,4,4′-Tetramethoxychalcone (α-Br-TMC) Inhibits the JAK/STAT Signaling Pathway (original) (raw)
Figure 4
RNA polymerase II recruitment to the Cis and Osm promoters is impeded in Ba/F3 cells treated with α-Br-TMC.
Ba/F3 cells were pre-treated 30 minutes with 0.2 µM TSA or 10 µM α-Br-TMC before being stimulated with 5 ng/mL IL-3 for 30 minutes. DMSO (vehicle) was adjusted to 0.02% in all conditions. Chromatin immunoprecipitation (ChIP) was performed as described in Materials and Methods using antibodies directed against STAT5 or RNA polymerase II (RNA Pol II) proteins. Co-precipitated genomic DNA was analyzed by quantitative PCR using primers specific for the STAT5 binding sites (STAT5 ChIP) or the transcription start site (RNA Pol II ChIP) of the mouse Cis (A) and Osm (B) genes.