YAP Promotes Ovarian Cancer Cell Tumorigenesis and Is Indicative of a Poor Prognosis for Ovarian Cancer Patients (original) (raw)
Figure 4
YAP promotes ovarian cancer cells migration and distant metastasis in vitro and in vivo.
A. Wound-healing assay for the migration of YAP-5SA and YAP-5SA-ΔC expressing cells after culture for 12 and 24 hours. Scale bar = 100 μM for all panels. B. Transwell assays for the migration of YAP-5SA and YAP-5SA-ΔC expressing cells. **, P<0.01. C. Bioluminescence imaging assays showing lung metastasis of control and YAP-5SA-ΔC expressing HO8910PM cells after they were injected into the caudal veins of nude mice. D. HE and IHC staining of lung metastasis sites shown in C. E-F: Images and statistical analysis results of liver metastasis by caudal vein injected HO8910 PM cells. G: HE and YAP IHC results of liver metastasis sites shown in E. H. Western blot results for EMT marker expressions in cultured YAP-5SA and YAP-5SA-ΔC expressing cells and in vivo xenografts derived from these cells.