Sequencing Degraded RNA Addressed by 3' Tag Counting (original) (raw)
Figure 1
Schematic drawing of the 3TC method.
Shown is a graphical representation of the annotation file used for counting. The original annotation file contains all annotated exons and isoforms for all genes and is shown on the left. The isoforms with the highest expression within a gene are indicated by a black borderline around its exons. The two steps of the 3TC process are shown in the middle; only those isoforms that have the highest expression are kept in the annotation file (isoform filtering) and all isoforms are truncated to a specific length N (length restriction, shown by shading). The final annotation file is shown on the right.