MicroRNA-27a Modulates HCV Infection in Differentiated Hepatocyte-Like Cells from Adipose Tissue-Derived Mesenchymal Stem Cells (original) (raw)
Figure 2
Expression of candidate HCV entry receptors in DHCs.
To evaluate the possible routes of HCV infection, the mRNA (A) and protein (B) expression of various HCV candidate receptors was assessed in DHCs. (A) RT-PCR analysis of the level of LDLR, CD81, SR-B1, and EGFR in Huh7.5 cells, AT-hMSCs, and DHCs. β-Actin was used as the loading control; Huh7.5 served as the positive control. (B) To detect the protein expression of candidate HCV entry receptors, the cells were stained with Dil-LDL (red), anti-CD81 antibodies (red), anti-SR-B1 antibodies (red), or anti-EGFR antibodies (red), and DAPI (blue). Merging of the images revealed strong expression of candidate HCV entry receptors in the DHCs. Scale bars are each 100 µm (LDLR), 50 µm (CD81 and SR-B1), and 200 µm (EGFR).