Melatonin Combined with Endoplasmic Reticulum Stress Induces Cell Death via the PI3K/Akt/mTOR Pathway in B16F10 Melanoma Cells (original) (raw)
Figure 2
Expression of p85α and p85β in B16F10 cells after melatonin with or without thapsigargin or tunicamycin treatment.
B16F10 cells were cultured with or without melatonin (0.1, 0.5, 1 mM) and/or thapsigargin (1 μM) for 6 hr or tunicamycin (5 μg/mL) for 16 hr. p85α and p85β expression was analyzed by Western blot (A). Relative amounts of p85α (B) and p85β (C) quantified as described in Materials and methods. Data are mean ± SD of three experiments. ***P<0.001 vs. untreated control; ##P<0.01, ###P<0.001, melatonin vs. melatonin with thapsigargin or tunicamycin.