Forced Resurgence and Targeting of Intracellular Uropathogenic Escherichia coli Reservoirs (original) (raw)
Figure 3
Chitosan treatment promotes the resurgence of UPEC reservoir populations into the bladder lumen.
Adult female CBA/J mice were inoculated with UTI89 via transurethral catheterization or left uninfected. After 3 d, phosphate buffer alone (control) or 0.01% chitosan was instilled into the bladders for 20 min prior to rinses with PBS. Bladders were bisected, splayed and imaged 1 or 7 d later, as indicated, using (A) confocal fluorescence microscopy or (B) SEM. Images in (B) show F-actin (purple), host nuclei (blue), and bacteria (yellow). Scale bars, (A) 50 μm and (B) 10 μm. Images are representative of three independent experiments.