In Vitro Selection and Characterization of New Probiotic Candidates from Table Olive Microbiota (original) (raw)
Figure 1
Experimental intestinal cell model settings used in this survey: (A) undifferentiated model with H4-1 epithelial cells grown on plastic surface; (B) functional 3D model where H4-1 were grown until the differentiation on microporous membrane; and (C) the complete 3D H4-1/TLT model with a differentiated layer of H4-1 in the apical compartment (1) and TLT monocyte/macrophages seeded in the basolateral side (2).
In the models (B) and (C) the polarization of H4-1 cells was obtained after 14–15 days of incubation with regular changes of the media (cells initially seeded at a density of 50 000 cells per cm2), and confirmed by measuring the TEER. In the model (C) inserts with polarized H4-1 cells were transferred into wells that had been underlain with TLT cells (200 000 cells each well and growth for 24–48 h).