The Antiviral Restriction Factors IFITM1, 2 and 3 Do Not Inhibit Infection of Human Papillomavirus, Cytomegalovirus and Adenovirus (original) (raw)
Figure 3
Ad5 and HCMV infections are not affected by overexpression of IFITM1, 2 and 3.
HeLa cells overexpressing IFITM1, IFITM2, or IFITM3 were infected with recombinant (A) Ad5-CMV-GFP or (B) HCMV TB40E mCherry and infectivity was measured by flow cytometry at 48 hpi. Gates were set based on the uninfected control and the percentage of fluorescent cells was quantified using FlowJo software (A & B). The results are presented from at least three independent experiments with standard error. (C) HeLa cells overexpressing IFITM proteins were infected with S-protein pseudotyped SARS-CoV luciferase viruses. Infectivity was measured by Bright-Glo Luciferase Assay System (Promega), normalized to the vector alone control, and shown as percent infectivity from triplicate samples representative of two independent experiments. _P_-values were calculated as described in Fig. 1. Significant differences (**p < 0.01) compared to vector-transduced cells are marked by asterisks.