IHC Profiler: An Open Source Plugin for the Quantitative Evaluation and Automated Scoring of Immunohistochemistry Images of Human Tissue Samples (original) (raw)
Figure 1
Representation of color deconvolution using the old and the new optical density (OD) vectors.
A: Color deconvolution using the old OD vectors. B: Color deconvolution using the new OD vectors. C: Scatter plot comparing the intensities on the complimentary image with the old OD vectors (blue) and the new OD vectors (red). D: Plot comparing the number of pixels with the intensity value of 255. An improvement between 2 to 10 fold is shown using 7 different samples. Each data plot represents an individual sample with its respective pixel count of the intensity value of 255.