Clinical Validation of an Ultra High-Throughput Spiral Microfluidics for the Detection and Enrichment of Viable Circulating Tumor Cells (original) (raw)
Figure 2
Enumeration of CTC from cancer patients.
(A) Immunofluorescence staining of isolated CTCs. CTCs (marked by white arrow) were identified by the following criteria: Hoechst+, pan-CK+ and CD45-. Scale bar: 20 µm (B) Box plot summary indicating the range of CK+cells/ml recovered from the sample outlet for blood samples extracted from healthy volunteers, as wells as breast and lung cancer patients. The box plot presents the median, lower and upper quartiles (25th,75th percentiles). Data points that lie outside the 10th and 90th percentiles are shown as outliers (Anova, p<0.001). Encapsulated image of PAP stained isolated cells shows a large CTC with high nucleus to cytoplasmic (N/C) ratio (labeled with white arrow). (C) Staining of CTC for pan-CK and CD45. Upper panel depicts a representative image of cells which were double positive (CK+/CD45+); while lower panel shows a double negative (CK-/CD45-) cell. Scale bar: 20 µm (D) Staining of CTC for pan-cytokeratin and EpCAM. Scale bar: 20 µm.