Long-Term Treatment with the Sodium Glucose Cotransporter 2 Inhibitor, Dapagliflozin, Ameliorates Glucose Homeostasis and Diabetic Nephropathy in db/db Mice (original) (raw)
Figure 5
Dapagliflozin inhibits oxidative stress in the kidney.
(A, B) ROS production was detected by fluorescence microscopy using dihydroethidium (DHE) staining. ROS was predominantly localized in the interstitia of db/db mice, and was suppressed in the db/db+dapa groups compared with that in the db/db group. Original magnification, ×100. Data are mean ± SEM. *P<0.05. (C, D) Localization of Nox4 was detected by immunohistochemistry. The expression of Nox4 was predominantly localized in the interstitia of db/db mice, and was suppressed in the db/db+dapa groups compared with that in in the db/db group. Original magnification, ×100. Data are mean ± SEM. *P<0.05.