Fgfr1 Inactivation in the Mouse Telencephalon Results in Impaired Maturation of Interneurons Expressing Parvalbumin (original) (raw)
Figure 2
Fgfr1 mutation does not alter the initial patterning of the ganglionic eminence and specification of the interneuron lineage.
In situ hybridization for Mash1 (A,B) and Nkx2.1 (C,D), in controls (A,C) and Fgfr1f/f;Nestin-Cre+ mutant mice (B,D), revealing that gene expression for these cell fate determinants for the PV neuron lineage was not disrupted by Fgfr1 mutation at E12.5. In situ hybridization for Gad1 demonstrated that GABA synthetic enzymes are appropriately expressed in Fgfr1 (F) mutants compared to controls (E) at E12.5. Immunofluorescence staining for the transcription factor Dlx2, also revealed equivalent staining in the MGE of control (G) and Fgfr1 mutants (H). Scale bar 20 µm in G and H.