Fgfr1 Inactivation in the Mouse Telencephalon Results in Impaired Maturation of Interneurons Expressing Parvalbumin (original) (raw)
Figure 7
Astrocyte-Interneuron co-culture experiments reveal altered cytoarchitecture for interneurons grown on astrocytes lacking Fgfr1.
Gad67-GFP+ positive interneurons were isolated from the MGE of E13.5 embryos wild type for Fgfr1, and cultured on astrocytes from Fgfr1f/f control (A, C) or Fgfr1f/f;hGFAP-Cre mice (B, D). Co-cultures maintained for 4 days had abundant numbers of GFP positive cells that appeared to be immature, with few processes (A,B, green = GFP, blue = s100beta). After two weeks in culture, interneurons developed many branched neurites, and appeared to prefer attachment to GFAP (red) positive areas of the culture dish. After two weeks in culture, no differences in the number of surviving interneurons were observed (E, averaged from 26 culture wells of 5 controls, and 12 culture wells of three Fgfr1 mutant mice). Representative Neurolucida drawings showing decreased complexity of interneurons grown on Fgfr1 mutant astrocytes as compared to those grown on control astrocytes (F and G). Cell perimeter (H) and the number of neurites per cell (I) were decreased based on neurolucida drawings of 60 neurons from 5 control samples, and 38 neurons from three Fgfr1 mutant samples. Axon length or the sum of dendrite lengths were not significantly different between the two groups (J).