Interacting Chemokine Signals Regulate Dendritic Cells in Acute Brain Injury (original) (raw)
Figure 6
Cyclophosphamide treatment in wt mice analyzed three days after TBI.
(a) Microarray showed markedly weakened injury-activated Ccr2 and H2-Aa levels while upregulation of the fractalkine receptor Cx3cr1 was not affected. (b) Itgax was also dampened in mice subjected to cyclophosphamide treatment as shown by qRT-PCR whereas Ccl3 upregulation was not impaired. (c) Flow cytometry demonstrated reduced number of brain cells gated for Cd11c in mice treated with cyclophosphamide. Isotype controls (upper middle panel) showed only trace labeling of inflammatory cells. Quantitative data from flow cytometry assays (right), show a substantial reduction of Cd11c-positive cDCs in the injured brains treated with cyclophosphamide compared to mice injected with saline.