Overexpression of Dyrk1A Is Implicated in Several Cognitive, Electrophysiological and Neuromorphological Alterations Found in a Mouse Model of Down Syndrome (original) (raw)
Figure 5
Normalization of the Dyrk1A copy number reduced the density of GABAergic and increased the density of glutamatergic synapse markers in the hippocampus of TS mice.
(a) Representative images of GAD65/67, VGLUT and GAD6567/VGLUT immunostaining. (b) Mean values ± SEM of the percentage of area occupied by GAD65/67- (top row) and VGLUT-positive boutons (middle row) in the hippocampus of TS +/+/+, TS +/+/− and CO +/+ mice and the ratio of these areas (lower row). GAD65/67: ANOVA ‘trisomy’: F(1,17) = 10.10, p = 0.006; ‘_Dyrk1A_’: F(1,17) = 3.91, p = 0.066. VGLUT: ANOVA ‘trisomy’: F(1,17) = 10.35, p = 0.006; ‘_Dyrk1A_’: F(1,17) = 4.49, p = 0.051; Ratio GAD/VGLUT: ANOVA ‘trisomy’: F(1,17) = 37.44, p<0.001; ‘_Dyrk1A_’: F(1,23) = 9.30, p = 0.009. **: p<0.01, ***: p<0.001 TS +/+/+ vs. CO +/+; #: p<0.05 TS +/+/+ vs. TS +/+/−.