Impaired Osteoblast Differentiation in Annexin A2- and -A5-Deficient Cells (original) (raw)
Figure 4
Effects of AnxA2 and AnxA5 knockdown on ALP and hydroxyapatite.
(A) ALP activity staining in MC3T3-E1 cells (MC3T3), Si, _AnxA2_kd and _AnxA5_kd cells after culture in osteogenic differentiation media for 7, 14 and 21 days, representative images from n = 3 biological replicates. (B) Quantitation of ALP staining intensity. Bars represent mean integrated signal intensity ± SEM, n = 3. + represents statistically significant difference from same genotype on day 0, p<0.05. **represented statistically significant different from Si at the same day, p<0.01. (C) OsteoImage staining for hydroxyapatite in Si, _AnxA2_kd and _Anx5_kd cells cultured with differentiation media for 5 weeks. Each bar represents OsteoImage fluorescence normalized to PI ± SEM, n = 3. ***represents statistically significant difference from maintenance media within the same genotype, ***p<0.001; + represents statistically different from Si in same media composition, p<0.01.