Regulation of Cilium Length and Intraflagellar Transport by the RCK-Kinases ICK and MOK in Renal Epithelial Cells (original) (raw)
Figure 6
Cilium length regulation by ICK and MOK interacts with cAMP and mTORC1 signaling.
(A) Average lengths of primary cilia of IMCD-3 cells expressing a control shRNA, ICKsh #01, or MOKsh #01, untreated or treated with 100 µM forskolin (Fsk) for 24 hours. (B) Immunofluorescence images of IMCD-3 cells expressing a control shRNA, ICKsh #01, or MOKsh #01, untreated or forskolin-treated, stained with anti-acetylated tubulin. (C) Average anterograde velocity of IFT20-GFP in IMCD-3 control cells, and cells depleted of ICK, untreated and forskolin-treated. (D) Average lengths of primary cilia of IMCD-3 cells expressing a control shRNA, ICKsh #01, or MOKsh #01, untreated or treated with 0.5 µM rapamycin (rapa) for 24 hours. (E) Immunofluorescence images of IMCD-3 cells expressing a control shRNA, ICKsh #01, or MOKsh #01, untreated or rapamycin-treated, stained with anti-acetylated tubulin. (F) Average anterograde velocity of IFT20-GFP in IMCD-3 control cells, and cells depleted of ICK, untreated and rapamycin-treated. Statistically significant differences (p<0.001) compared to untreated cells are indicated with a black asterisk, and compared to the control shRNA are indicated with a red asterisk. Error bars indicate SD. Numbers in the bars indicate number of cilia (A and D) or particles (C and F) analyzed. Scale bar, 10 µm.