Hydroxytyrosol Prevents Increase of Osteoarthritis Markers in Human Chondrocytes Treated with Hydrogen Peroxide or Growth-Related Oncogene α (original) (raw)
Figure 1
Hydroxytyrosol reduces the levels of ROS in chondrocytes treated with H2O2 or the chemokine GROα.
Monolayer cultures of OA chondrocytes, loaded with the fluorogenic probe DCF-DA as detailed in Methods, were pre-incubated in the absence or in the presence of HT for 30 min before addition of H2O2 (A) or GROα (B). After 60 min, cells and conditioned medium were separated and assayed for reactive chemical species. Data are means ± S.E.M. of 6 (A) or 4 (B) separate determinations; ** p<0.01 and *** p<0.001 vs. cells treated only with H2O2 or GROα. Interestingly, comparison of all control cells and cells treated only with HT (A+B) showed a significant difference between means (p<0.05).