Hydroxytyrosol Prevents Increase of Osteoarthritis Markers in Human Chondrocytes Treated with Hydrogen Peroxide or Growth-Related Oncogene α (original) (raw)
Figure 4
Hydroxytyrosol prevents the expression of OA-related genes in chondrocytes treated with H2O2.
Monolayer cultures of OA chondrocytes were pre-incubated in the absence or in the presence of HT for 30 min before addition of H2O2. At the time indicated after H2O2, cells were harvested and analysed by qRT-PCR for the amount of COX-2 mRNA (A), iNOS mRNA (B), MMP-13 mRNA (C), RUNX-2 mRNA (D), VEGF mRNA (E) and SIRT-1 mRNA (F). Data are means ± S.E.M. of (n) separate determinations; * p<0.05, ** p<0.01 and *** p<0.001 vs. cells treated only with H2O2. Other comparisons between means showed not significant differences.