Hydroxytyrosol Prevents Increase of Osteoarthritis Markers in Human Chondrocytes Treated with Hydrogen Peroxide or Growth-Related Oncogene α (original) (raw)
Figure 6
Hydroxytyrosol regulates the expression of OA-related genes in 3-D cultures of chondrocytes treated with GROα.
OA chondrocyte micromasses at 1, 2 and 3 weeks maturation were pre-incubated in the absence or in the presence of HT for 30 min before addition of GROα as described in Methods. Then micromasses were harvested and analysed by qRT-PCR for the amount of COX-2 mRNA (A), iNOS mRNA (B), MMP-13 mRNA (C), RUNX-2 mRNA (D), VEGF mRNA (E) and SIRT-1 mRNA (F). Data are means ± S.E.M. of (n) separate determinations; * p<0.05, ** p<0.01 and *** p<0.001 vs. cells treated only with GROα. Other comparisons between means showed not significant differences, except for Control vs. HT+GROα at 1 w (* p<0.05) and at 2 w (*** p<0.001) in panel F (SIRT-1 mRNA).