Interleukin-34 Restores Blood–Brain Barrier Integrity by Upregulating Tight Junction Proteins in Endothelial Cells (original) (raw)
Figure 4
IL-34 upregulates tight junction proteins in MBEC4 cells.
MBEC4 cells were incubated with TNF-α (20 ng/ml) and IL-1β (20 ng/ml) in the presence of IL-34 (0–100 ng/ml) and GW2580 (1 µmol/L) for 24 h. Upper, representative images of Western blots for tight junction proteins. Bottom, quantified expression levels of tight junction proteins relative to those in untreated cells. Values are means ± SEM (n = 5). *, p<0.05.