A Rapid Optical Clearing Protocol Using 2,2′-Thiodiethanol for Microscopic Observation of Fixed Mouse Brain (original) (raw)

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Figure 5

Images of dendritic spines along a single hippocampal neuron.

(a) Combination of TDE treatment and water/oil-immersion objective lens with a high NA for imaging dendritic spine shapes in deep (100 µm) regions in a fixed brain slice. (b) Connected images of dendritic spines along single pyramidal neurons extending from a depth of 30 to 100 μm from the surface of the hippocampal slice. The left inset shows a low-magnification image of the hippocampus, and the circle shows the observed neuron. (c-h) Magnified images of the dendritic spine shapes on the basal dendrite (c-e) and the apical dendrite (f-h) along the neuron shown in (b). All images are maximum projection images.

Figure 5

doi: https://doi.org/10.1371/journal.pone.0116280.g005