Inhibition of Bone Morphogenetic Protein Signal Transduction Prevents the Medial Vascular Calcification Associated with Matrix Gla Protein Deficiency (original) (raw)
Figure 5
BMP signaling is not increased in aortas of MGP-/- mice.
(A) Protein lysates were isolated from the aortas of 7-, 14-, and 28-day-old WT and MGP-/- mice. Each lane represents protein isolated from four pooled aortas. PVDF membranes were incubated with antibodies directed against phosphorylated Smad 1/5 (P-Smad 1/5) and total Smad 1. The ratio of P-Smad 1/5 to total Smad 1 was the same in aortas derived from WT and MGP-/- mice. (B) RNA was isolated from aortas of WT and MGP-/- mice at 1, 7, 14, and 28 days of (n = 6–8 in each group, as indicated). No difference in aortic Id1 mRNA levels was observed between MGP-/- and WT mice.