Application of VEGFA and FGF-9 Enhances Angiogenesis, Osteogenesis and Bone Remodeling in Type 2 Diabetic Long Bone Regeneration (original) (raw)
Fig 3
Reduced angiogenesis in db-/db- mice tibia defects.
(A) Blood vessels and endothelial cells were detected by fluorescence immunohistochemistry for PECAM-1 (3dpO). _db_-/_db_- mice showed less angiogenesis compared to WT mice. (B) Higher magnifications from (A) (white box). (C) Quantification of PECAM-1 positive pixels (arbitrary units, a.u.) revealed that angiogenesis is significantly reduced in _db_-/_db_- mice compared to tibiae of WT mice. Results are shown as ± SEM. P-value: ** < 0.01 (two sample t-test). Scale bars: A: 200 μm, B: 45 μm.