The Effects of Secretion Factors from Umbilical Cord Derived Mesenchymal Stem Cells on Osteogenic Differentiation of Mesenchymal Stem Cells (original) (raw)
Fig 1
hUCMSCs secretion factors promote mineralization in osteogenic differentiation of hBMSCs.
(A) hBMSCs were treated with hUCMSCs secretion factors (from 0.01 μg/ml to 20 μg/ml) for 3 days or 5 days. MTT assay was performed to test the cell proliferation rate. DMSO served as control. The hUCMSCs secretion factors have no significant effect on cell proliferation. (B) The dose-dependent assay of hUCMSCs secretion factors (5, 10 and 20 μg/ml) on osteogenic differentiation of hBMSCs that were treated for 11 days in 24-well plates. Calcium deposits formation was rare in the 5 μg/ml or 10 μg/ml group, whereas significant calcium nodules were formed in the 20 μg/ml group. (C) Time-dependent assay of hUCMSCs secretion factors at a dose of 20 μg/ml on osteogenic differentiation of hBMSCs that were treated for 3, 5, 7 and 11 days in 24-well plates. Alizarin Red staining showed that the amount of calcium deposits in both hUCMSCs secretion factors and OIM + hUCMSCs secretion factors groups were higher than those of the OIM group at all the time points. (D) Alizarin Red was dissolved in 10% (w/v) cetylpyridinium chloride, and the amount was determined by absorbance measurement at 570 nm. The amount of Alizarin Red in both hUCMSCs secretion factors and OIM + hUCMSCs secretion factors groups was significantly higher than that in the control group at day 3, 5, 7 and 11. (OIM: osteogenic induction medium. hUCMSCs secretion concentration: 20 μg/ml * p<0.05, compared to control).