The Effects of Secretion Factors from Umbilical Cord Derived Mesenchymal Stem Cells on Osteogenic Differentiation of Mesenchymal Stem Cells (original) (raw)
Fig 2
hUCMSCs secretion factors up-regulated expression of osteogenic marker genes of hBMSCs.
hBMSCs were incubated with 20 μg/ml hUCMSCs secretion, osteogenic induction medium and osteogenic induction medium with 20 μg/ml hUCMSCs secretion for 11 days before RNA extraction and real time qRT-PCR. The expression of ALP, BMP2, Col1α, OCN, Osterix and Runx2 was significantly increased in the hUCMSCs secretion factors group, OIM group and OIM + hUCMSCs secretion factors group compared to that of the control group. Cells treated with α-MEM medium served as a control. (* p<0.05, compared to control)