miR-185 Plays an Anti-Hypertrophic Role in the Heart via Multiple Targets in the Calcium-Signaling Pathways (original) (raw)
Fig 5
miR-185 negatively regulates the activity of CaMKIIδ in NRVMs.
(A and B) 72 h after transfection of miR-185 mimic or miR-185 inhibitor, the level of p-CaMKIIδ and total CaMKIIδ were analyzed by western blotting. GAPDH was used as a loading control. (C) Western blotting showing p-PLB (Thr-17) and PLB protein expression in NRVMs transfected with NC or miR-185. 24 h after transfection, NRVMs were stimulated with ET-1 (10 nmol/L) for 48 h. The blots were stripped for 30 min and reprobed with PLB for loading control. The data are expressed as mean ± SEM; *P < 0.05, **P < 0.001, N = 3.