Berberine Inhibits Invasion and Metastasis of Colorectal Cancer Cells via COX-2/PGE2 Mediated JAK2/STAT3 Signaling Pathway (original) (raw)
Fig 1
Berberin inhibits CRC cell growth and metastasis.
A. Berberin inhibits the CRC xenograft tumor growth. Subcutaneously implanted tumors of CRC cell line SW620 and LoVo were treated with control, 5-FU, and 3 dosages of berberin for 28 days. Tumor volumes of different groups of mice were recorded and presented. B. Xenograft tumors excised from the mice treated with drugs and duration indicated in A. C. Berberin decreases CRC cell viability in vitro. SW620 and LoVo cells were treated with variable concentrations of berberin for 24, 48 and 72 h. The mean value ± SE from 5 replicates was shown. D. Berberin decreases CRC cell lung metastasis in mice. Mice bearing SW620 and LoVo cell lung metastasis were treated with indicated variable berberin dosages. Upper panel: representative HE stained sections of mouse lung with CRC cell metastasis foci. Lower panel: numbers of CRC lung metastasis foci in control and berberin treated mice. E. Berberin inhibits migration and invasion of CRC cells. Cell migration and invasion assay results of CRC cell lines SW620 and LoVo, treated with berberin or control. The results shown are representative of three independent experiments. The bars and error bars represent the means ± SE, and the _P_-value was calculated using an independent _t_-test. **P<0.01, control vs treated; ##P<0.01, control vs treated.